HPLC METHOD DEVELOPMENT BLUEPRINT

COURSE DETAILS:

✅The "HPLC Method Development Blueprint" course is a comprehensive training program designed to master HPLC technique. This course offers an in-depth exploration of the working principles of various LC techniques, guiding participants through the critical process of HPLC method development.

✅It covers the selection criteria for HPLC columns based on interaction, dimension, pore size, and ligand, especially for small molecules, proteins and peptides, mobile phase selection- including solvents, buffers, and pH levels.

✅The role of pH and pKa in method development is thoroughly examined, including methods for determining pKa through UV spectroscopy and potentiometry.

✅The course addresses common challenges in developing methods for related substances, gradient elution, and strategies to overcome problems like peak splitting, column degradation, and carryover effects. Special attention is given to the principles of chiral separation and the use of protein-based columns for this purpose.

✅The course also includes practical tips for achieving a smooth baseline during gradient runs and retaining acidic and basic compounds effectively.

✅The course features live session recordings from various dates, providing real-life examples and case studies. These sessions, including those focusing on QbD & AQbD, offer participants a unique opportunity to see HPLC method development in action, discuss common issues, and learn from experienced practitioners.

✅CERTIFICATION COURSE: Receive a certificate upon successfully finishing this course

✅DURATION: 1268 Minutes (21.13 Hours)

✅ LANGUAGE: English

✅ ONLINE COURSE: Offered as an online course featuring pre-recorded videos and structured for distance learning, this program is available to participants globally.

✅LIFETIME ACCESS: You will have lifetime access to the course